Structural Molecular Biology

BL14-1

Beam line 14-1 is a bending magnet side station. It is dedicated for monochromatic, high-throughput macromolecular crystallography and optimized for SAD and MAD experiments. It can be run in a full remote access mode. It is equipped with a Rayonix MX325 CCD detector.

See the Macromolecular Crystallography website for technical details.

BL12-2

Beam line 12-2 is a PRT station, realized through third party funding from the Gordon and Betty Moore Foundation via the California Institute of Technology and available to general users 70%; it is a 134-pole, 1.0-Tesla in-vacuum undulator beam line with fully adjustable focus from 100 to 15 microns. smaller beams can be achieved by the use of microcollimators. It is optimized for microdiffraction, monochromatic, high-throughput and high-resolution macromolecular crystallography. It is SAD and MAD capable and can be run in a full remote access mode.

BL12-1

Beam line 12-1 employs a 154-pole, 1-Tesla in-vacuum undulator, a flat side-deflecting Rh-coated Si mirror, and a liquid nitrogen-cooled double-crystal Si(111) monochromator with a 0.01% energy bandpass. The beamline is also fitted with a multilayer with a ~1% energy bandpass that boosts the x-ray flux by a factor by about two orders of magnitude.. Kirkpatrick-Baez (KB) optical mirrors are used for 18:1 horizontal and 12:1 vertical beam focusing. The vertical mirror polish is ~100 nrad RMS and a vertical beam focus of ~5 µm FWHM.

BL9-3

Beam line 9-3 is a wiggler side-station dedicated to biological x-ray absorption spectroscopy and EXAFS measurements of dilute solutions and single crystals. BL9-3 is equipped with a 100-element Ge monolithic solid-state detector in addition to ionization chambers and PIPS/Lytle detectors. A dedicated LHe cryostat allows for routine low temperature measurements on solutions. A Huber-Kappa goniometer, a LHe cryostream, a focusing polycapillary and a MAR CCD detector are available (in addition to specialized software) for single crystal measurements.

BL9-2

Beam line 9-2 is a wiggler end station dedicated for monochromatic, high-throughput and high-resolution macromolecular crystallography and optimized for SAD and MAD experiments. It can be run in a full remote access mode. It is equipped with a Dectris Pilatus 6M PAD detector and a remote access controlled UV-Vis microspectrophotometer.

See the Macromolecular Crystallography website for details.

BL8-2

Beam line 8-2 can be used to probe a wide range of core levels using photoemission and x-ray absorption spectroscopies. At BL 8-2, currently only one end-stations is available for the general user program. The first end-station is a high throughput setup for soft x-ray spectroscopy measurement – upgrades in progress include a fixed position multipurpose chamber with a load lock system for high throughput sample loading, capable of probing a wide range of materials with absorption and photoemission techniques.

BL7-3

Beam line 7-3 is a wiggler side-station dedicated for x-ray absorption spectroscopy and EXAFS measurements on dilute biological systems. BL7-3 is equipped with a 30-element Ge solid-state detector in addition to ionization chambers and Lytle/PIPS detectors. A dedicated LHe cryostat allows for routine low temperature measurements. The unfocused beam on BL7-3 is ideal for samples especially susceptible to radiation damage, such as high-valent intermediates and other oxidized species.

BL7-1

Beam line 7-1 is a 8-pole, 2.0-Tesla wiggler side-station beam line optimized for monochromatic, high-throughput, high-resolution macromolecular crystallography. It is SAD and MAD capable and can be run in a full remote access mode. It is equipped with an ADSC Q315R CCD detector. Currenlty 7-1 is dedicated to staff development and user training.

See the Macromolecular Crystallography website for details.

BL4-1

Beam line 4-1 is a high-flux station optimized for x-ray absorption spectroscopy and EXAFS experiments requiring x-rays with energies between ~ 6 and 38 keV.  This energy range includes most of the transition metals (all rows), lanthanides and actinides, P-block elements, alkaline and alkaline earths. While it is possible to access x-rays as low as 5 keV, these experiments are challenging because of air absorption and are better performed at BL4-3.

BL4-2

Beam Line 4-2 is a permanent experimental station for small-angle X-ray scattering and diffraction (SAXS) techniques dedicated to research in structural biology and biophysics. The station provides state-of-the-art experimental facilities for structural studies on biological material such as nucleic acids, proteins, protein assemblies, virus particles, biological fibers as well as lipid membranes and membrane-protein/DNA complexes.