BL12-1

Overview
SPEAR3

Micro-focus Macromolecular Crystallography 154-pole In-Vacuum Undulator End Station

Beam line 12-1 employs a 154-pole, 1-Tesla in-vacuum undulator, a flat side-deflecting Rh-coated Si mirror, and a liquid nitrogen-cooled double-crystal Si(111) monochromator with a 0.01% energy bandpass. The beamline is also fitted with a multilayer with a ~1% energy bandpass that boosts the x-ray flux by a factor by about two orders of magnitude.. Kirkpatrick-Baez (KB) optical mirrors are used for 18:1 horizontal and 12:1 vertical beam focusing. The vertical mirror polish is ~100 nrad RMS and a vertical beam focus of ~5 µm FWHM.

BL12-1 is a preeminent macromolecular crystallography capability for structural biology research in the US and is designed for investigations of the most challenging structural investigations of biological systems confronting scientists today, including cases where crystals can only be grown to a few microns in size. BL12-1 is competitive with the performance of the best in class micro-beam instruments in the US (e.g., the native vertical focus of 5 µm and a collimated beam size of 1.5 µm2 produces a flux density > 6x that of SSRL BL12-2).

BL12-1 has improved performance at low energy and is equipped with an Eiger 16M pixel array detector with readout speeds over 100 Hz full detector and over 750 Hz for regions of interest which enables ultra-high-redundancy SAD experiments and routine serial crystallography experiments using liquid jets.

BL12-1 is also an effective gateway for the Linac Coherent Light Source (LCLS), providing a similar platform to the LCLS-MFX instrument for equipment R&D, testing, prescreening and characterization of micro- to nano-crystalline samples prior to LCLS studies.

See the Macromolecular Crystallography website for technical details.

Sample Environment —

Sample temperature 100K - elevated temperatures or room temperature at controlled humidity
Other Cryogenic Temperatures (Oxford Cryo-cooler)
Elevated Temperature (Oxford Cryo-cooler)
Controlled Humidity (Arinax Humidity Control Device)

Instrumentation —

Detector Dectris EIGER 2XE shutterless data collection, fine phi slicing experiments, serial diffraction
Goniometer Single-axis micro-diffractometer, air bearing, coaxial cold stream, 1.5 micron SOC
Sample Exchange Robot Stanford Automated Mounter compatible with magnetic sample bases inside
Other SSRL cassettes, Universal Pucks or SSRL in-situ plates
Stanford Automated Mounter

Publications

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Science Highlights

Beam Line 12-1 Science Highlights

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