Experimental Station 12-2

Beam line 12-2 is a PRT station, realized through third party funding from the Gordon and Betty Moore Foundation via the California Institute of Technology and available to general users 60-80%; it is an undulator beam line with fully adjustable focus from 100 to 15 microns. Micron-sized beams  can be achieved by the use of microcollimators.  It is optimized for  microdiffraction, monochromatic, high-throughput and high-resolution macromolecular crystallography.  It is SAD and MAD capable and can be run in a full remote access mode.  It is equipped with an Dectris PILATUS 6M detector. For aditional information about the experimental capabilities, see http://smb.slac.stanford.edu/index.shtml.

Status
Open
Supported Techniques
Macromolecular Crystallography
Multi wavelength anomalous diffraction (MAD)
Main Scientific Disciplines
Biomedical Sciences
Bioscience
Structural Molecular Biology
Beam Line Specifications

Source

134-pole, 1.0-Tesla in-vacuum undulator, 0.1 mrad acceptance

Source In-vacuum mini-gap undulator, end station

  Energy Range Resolution ΔE/E Spot Size Flux
Focused 6.69-17.0  keV ~3 x 10-4 H: 0.015-0.2 mm
W: 0.05-0.10 mm
3.3 x 1012

See also: BioSync 12-2

Optics

Mirror: Flat Si Rh coated M0, Kirkpatrick-Baez flat bent Si M1 & M2

Monochromator: Liquid nitrogen-cooled double crystal Si(111)

Absorption

N/A

Instrumentation

Detector:  Dectris Pilatus 6M, pixel, sample to detector distance of 188-900 mm, shutterless data collection, fine phi slicing experiments

Goniometer: SSRL single-axis microdiffractometer, air bearing, coaxial cold stream, 1.5 micron SOC

Sample to Detector Distance: 188-900 mm

Cryo Capability: Oxford Cryojet

 

Sample Environment

Ambient pressure. 

Sample temperature: 100K - room temperature

Data Acquisition and Analysis

The beam line control GUI Blu-ice is installed at all the macromolecular crystallography beam lines. Web-ice provides an interface for diffraction image viewing and automated autoindex and data collection strategy calculation.

Other supported software installed on servers accessible from all the beam lines include mosflm, HKL2000 and XDS. MAD/SAD scripts, developed at SSRL, are also available to facilitate on-line structure solution. The graphics package COOT is installed on the local beamline computers.

Beam Line Phone
650-926-5212. Support cell phone: 650-714-9405
Beam Line Contact

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