Advances in X-ray Scattering/Diffraction Studies on Non-Crystalline Biological Systems
The significance of non-crystalline diffraction techniques has been rediscovered in recent years by an increasing number of structural biologists to complement higher resolution structures by crystallography, NMR and cryo-EM. The latest advances in X-ray solution scattering, fiber and membrane diffraction studies on biological systems will be reported by several experts in a diverse spectrum of structural biology benefiting from non-crystalline diffraction studies. The workshop, as a part of the 32nd SSRL Users' Meeting, will include demonstrations of several computer programs for processing solution x-ray scattering and interpreting low-resolution structures. The participants will also receive updates on current and future developments at SSRL BL4-2, the dedicated small angle scattering/diffraction facility for structural biology, funded by NIH NCRR and DOE OBER.
Organizer: Hiro Tsuruta (SSRL), tsuruta@slac.stanford.edu
Confirmed speakers:
Sebastian Doniach (Stanford University)
Keith O. Hodgson (SLAC/Stanford University)
Huey Huang (Rice University)
John E. Johnson (Scripps Research Institute)
Gerald Stubbs (Vanderbilt University)
Dmitri Svergun (EMBL Hamburg, Germany)
Annette Tardieu (Lab. PBSF, Paris, France)
Hiro Tsuruta (SSRL)
Willy Wriggers (University of Texas Health Science Center at Houston)
Naoto Yagi (SPring-8, Hyogo, Japan)
Please register at the SSRL users conference web site.
The participation in the software practical sessions requires your own laptop computers (Windows PC preferred).
Inquires should be made to the workshop organizer Hiro Tsuruta, tsuruta@slac.stanford.edu
Preliminary program (Titles and time lines tentative)
| Saturday October 15, 2005 (ROB Bldg., Redwood Room A/B) |
| 8:00 | Registration and morning refreshments |
| 8:30 | Keith Hodgson: SSRL SMB program |
| 8:45 |
Hiro Tsuruta- Quick survey of SAXS/D studies in biology |
| 9:00 |
Dmitri Svergun- Solution scattering data interpretation |
| 10:00 | Break |
| 10:15 |
Sebastian Doniach- Nucleic acid solution structure |
| 11:15 |
J.E. Johnson- Interplay with crystallography and cryo-EM in virus research |
| 12:15 | Lunch |
| 13:15 | Annette Tardieu - Solution scattering: bio-molecular interactions and crystallization |
| 14:15 |
Huey Huang- Protein-induced structures in membranes: pore and fusion |
| 15:15 | Break |
| 15:30 |
Gerald Stubbs- Fiber diffraction studies on filamentous viruses |
| 16:30 |
Naoto Yagi- Non-crystalline diffraction studies at a 3rd generation SR facility |
| 17:30 | Poster session (tent) |
| 19:00 | Workshop dinner (off site) |
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| Sunday October 16, 2005 (ROB Bldg., Redwood Room A/B) |
| 8:00 | Morning refreshments |
| 8:30 | BL4-2 biological SAXS/D facility updates |
| 9:00 | Tour of BL4-2 and software/hardware demonstration |
| 10:45 | Break |
| 11:00 | ATSAS software demonstration part I (Svergun) |
| 12:00 | Lunch |
| 13:00 | ATSAS software demonstration part II (Svergun) |
| 14:00 |
Willy Wriggers: Using Situs for the registration of protein structures with SAXS bead models. |
| 15:00 | Break |
| 15:15 | Situs demonstration (Wriggers) |
| 16:00 | Practical sessions and questions/answers |
| 18:00 | End of workshop
Post workshop and pre-User Meeting reception
(Panofsky Auditorium foyer/breezeway)
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