Experimental Station 4-2

Beam line 4-2 is a small-angle scattering/diffraction station dedicated to structural biology studies primarily on non-crystalline systems. The instrument covers the range of characteristic length scale of a few Angstroms to a few micrometers by the combined use of a pin-hole geometry camera, which can be automatically configured to have one of 7 sample-to-detector distances, and a separate ultra small angle scattering setup. A Rayonix MX225-HE area detector is the primary detector. For fast Time-resolved studies there is also a Dectris Pilatus 300K pixel array detector system available. The facility provides a large selection of special sample handling devices, such as stopped-flow rapid mixers, capillary flow cells and automated sample changer for solution scattering among others.

Status
Open
Supported Techniques
Biological Small-angle X-ray Scattering (BioSAXS)
Fiber diffraction
Lipid membrane diffraction
Small-angle single crystal diffraction
Main Scientific Disciplines
Bioscience
Beam Line Specifications

Source

20-pole, 2.0-Tesla Wiggler ID End Station

  Energy Range Resolution ΔE/E Spot Size Flux (ph/sec)
Focused
(Crystal)
6000-17000 eV 5 x 10-4 0.2 x 1mm2 3 x 1012
Focused
(Multilayers)
8000-12000 eV 3 x 10-2 0.2 x 1mm2 1 x 1014

 

Optics

M0 mirror: bent cylinder

15.4m vertical radius (adjustable) and 57 mm horizontal radius

Rh-coated silicon (cutoff energy 17.5 keV)

mean angle of incidence: 3.8 mrad

Magnification 1.0, upward reflecting 

Monochromator(s): liquid N2 cooled Si(111) and Mo/B4C multilayer

Absorption

N/A

Instrumentation

Detectors: Rayonix MX225HE, Dectris Pilatus 300k (for fast TR-SAXS), photodiode for transmitted intensity, APD for incident intensity, Vortex detector

Slits: JJ-Xray beam defining slits, Xenocs scatterless guard slits         

Other: fast shutter and variable filters, mirror pitch feedback for beam stability, variable length flight path

Angular range (at 11 keV, 4 mm beamstop):

Distance(m) Typical q -Range (Å-1)
0.25 0.045 - 3.6
0.45 0.024 - 2.2
0.65 0.017 - 1.6
1.10 0.010 - 1.0
1.65 0.0068 - 0.67
2.40 0.0045 - 0.46
3.40 0.0034 - 0.33

Please refer to http://ssrl.slac.stanford.edu/~saxs

Sample Environment

Autosampler (automated liquid sample loader):

Sample volume: 10 ul – 30 ul

Temperature range: 5 C - 40 C

Manual loading (flow) cell :

Sample volume: > 5 ul

Temperature range: 5 C – 70 C

Stopped-flow (for fast time-resolved solution SAXS) :

Sample volume: 30 ul-100 ul (per shot)

Temperature range: 5 C – 70 C

Mixing deadtime: ~1 ms

Humidity Chamber (for lipid diffraction and GiSAXS) :

Sample temperature range: 10 C – 50 C

rH range: 30% - 100%

Data Acquisition and Analysis

Data acquisition: Blu-ice, HiPic 
Data analysis: SasTool, SAXSPipe, ATSAS suite, Graphit 

see: http://www-ssrl.slac.stanford.edu/~saxs/daq/daq.htm 

X-ray Data Booklet

Beam Line Phone
650-926-5242. On-site Users: Contact the Duty Operator at 9-926-4040
Beam Line Contact
Beam Line Engineering Notes

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