BL12-1: A New Microfocus Beamline to Study the Most Challenging Biological Systems   Commissioning Phase

BL12-1 will be a preeminent macromolecular crystallography capability for structural biology research in the US and has been designed for investigations of the most challenging macromolecular crystallography systems confronting scientists today, including cases where crystals can only be grown to a few microns in size.  BL12-1 will be competitive with the performance of the best in class micro-beam instruments in the US (e.g., the native vertical focus of 5 µm and a collimated beam size of 1.5 µm2 will produce a flux density >6x that of SSRL BL12-2).  BL12-1 will have improved performance at low energy and will be equipped with an Eiger 16M pixel array detector with readout speeds up to 133 Hz full detector and 750 Hz for regions of interest which will enable ultra-high-redundancy SAD experiments and routine serial crystallography experiments using liquid jets.  BL12-1 will be an effective gateway for the Linac Coherent Light Source (LCLS), providing a platform for instrument R&D, prescreening and characterization of micro- to nano-crystalline samples prior to LCLS studies. BL12-1 is currently in the optics commissioning phase.


Status Commissioning
Percentage General Use 50%
Flux (p/s) 1e+13 (ʎ = 0.98 Å)
Multilayer Flux (p/s) 1e+14 (ʎ = 1.55 Å)
Multilayer Energy Band Pass (%) 1%
Experiment Types Monochromatic, MAD, SAD, Microbeam, Jet
Energy Range (keV) 6 - 18
Wavelength Range (A) 0.69 - 2.1
Max Resolution (A) * 0.69
Maximum Resolution Offset (A) 0.52
Min Beam Size (um) * 5
Max Beam Size (um) * 50
Detector Eiger 16M
Detector Technology PAD
Detector Size (mm x mm) 311 x 328
Detector Pixel Size (um) 75
Sample to Detector Range (mm) * 50 - 850
Dead Time (ms) 2e-3
Max Image Rate (Hz) * 133
Optimal Image Rate (Hz) 10
Max Detector Horizontal Offset (mm) 50
Max Detector Vertical Offset (mm) 220
PRTs Stanford, The Scripps Research Institute



Auto-Data Processing Web-Ice
Auto-Drug Pipeline * Drone
Data Collection Stategy Web-Ice
Micro-Beam BL12-1 Configuration
Remote Access NXClient
Sample Annealing Cryo-Cooler Axial Mount
Sample Screening SAM Robot
Sample Storage Long Term Storage Dewar Farm
Sample X-ray Rastering Blu-Ice

Additional On-Site Features


Sample Ambient-Temperature * Ambient Sample Trays
Sample Cassette Loading Cryo-Tools and SAM Cassette Kit
Sample Freezing In-Situ Cryo-Nozzle Axial Mmount
Sample Gas Pressurization Gas Pressurization Cell (Kr or Xe)
Sample Injection * MESH Jet
Sample Injection Viscous * LCP jet
Sample Magnification Standard Microscope
Sample Microcrystal Magnification High Resolution Microscope
Sample Prep Wet Lab
Sample Prep and Storage Cold Room
Sample Storage Short Term Incubator