Given that GCC185 plays vital roles both in organizing the cell cytoskeleton and in vesicle traffic, Schweizer Burguete et al. investigated how the putative tether itself is localized to the Golgi membrane. We have shown that Golgi-recruitment of GCC185 is mediated by the cooperation of two Golgi-localized small GTPases belonging to the Rab and Arf families. Rab6 binding to GCC185 promotes the subsequent binding of Arl1 to an immediately adjacent domain. Biochemical analysis of these interactions revealed a helical, dimeric, Rab6 binding domain in GCC185. The crystal structure of a complex between the Rab binding domain (RBD) of GCC185 and Rab6 was determined using diffraction data obtained at SSRL (Beamlines 11-1 and 7-1) and provided the stoichiometry and the molecular details of this interaction. Rab6 switch I and II regions, which adopt a specific conformation when the protein is GTP-bound, contact a dimeric coiled-coil in GCC185 with two-fold symmetry, and residues critical for Rab binding and Golgi-localization of GCC185 lie in the binding interface (Figure 1).

Based on our observations we have created a structure-derived model for simultaneous GTPase binding to the carboxy-terminal region of GCC185. In this model the three proteins form a hetero-hexameric complex that attach the 185 kDa tether to the surface of Golgi membranes (Figure 2). This model highlights how Arf and Rab-family members may interact with the same binding partner at different distances from the membrane. The Rab GTPases are expected to reach binding sites as far as 10 nm away from the membrane via their unstructured and membrane-anchored, C-terminal tails. Arf GTPases on the other hand, will bind to membrane-proximal domains, enabling cooperation with Rab proteins in determining the fate of a common binding partner.

	Figure 2. Model for dual GTPase binding to adjacent RBD and GRIP domains in GCC185. Rab6 (blue) and Arl1 (gray) anchor GCC185 (green) to Golgi membranes by inserting prenyl and myristoyl groups (gray stick models) respectively into the cytosolic leaflet of the lipid bilayer (beige). This model was generated by combining the Rab6-GCC185 crystal structure with that of a modeled Arl1-GCC185 GRIP domain complex. Regions absent from the two crystal structure models are shown in red.