Registration and all lectures will take place in the Redwood rooms (building 48). Lunch and dinner will also be served in building 48, unless noted otherwise. The practical tutorials will take place at different locations around the SSRL and experimental floor.

Monday 4 May

From Gene Synthesis to Crystallization workshop

• 7:30 - 8:30   Registration

• 8:30 - 10:30   Lectures: Crissy Tarver

• 10:30 - 11:00   Coffee Break

• 11:00 - 12:30   Hands-on Session 1

• 12:30 - 13:30   Lunch (Redwood rooms patio)

• 13:30 - 15:00   Hands-on Session 2

• 15:00 - 15:30   Coffee Break

• 15:30 - 17:00   Hands-on Session 3

• 17:15 - 18:45   Hands-on Session 4

• 19:00 - 20:00   Welcome Social (Redwood rooms patio)

Tuesday 5 May

Lectures in crystal handling, data collection, detectors and data reduction

• 8:30 - 9:00   Registration

• 9:00 - 9:30   Introduction to RapiData: Clyde Smith

• 9:30 - 10:15   Macromolecular Cryocrystallography: Some Opinions About Best Practices: Jim Pflugrath

• 10:15 - 10:30   Coffee Break

• 10:30 - 11:15   Getting the Best Data from Photon Counting Detectors: TBA

• 11:15 - 12:00   Radiation Damage: Elspeth Garman

• 12:00 - 13:00   Lunch (Redwood rooms patio)

• 13:00 - 13:45   Data Processing Fundamentals: Harry Powell

• 13:45 - 14:30   Room and Elevated Temperature Data Collection: New Tools and Automation: Silvia Russi

• 14:30 - 15:15   Data collection at Free Electron Lasers: Nick Sauter

• 15:15 - 15:30   Coffee Break and Group photo

• 15:30 - 16:30   Orientation to Experiments /SSRL Tour (building 120)

• 17:00 - 17:30   Introduction to the Tutorials: Clyde Smith

• 17:30 - 18:30   NX Client installation and remote desktop workshop: Darya Marchany

• 18:45 - 19:45     Banquet Dinner (Redwood rooms patio)

Wednesday 6 May

Lectures on structure solution and complementary methods

• 8:00 - 8:45   SAD and MAD: Ana Gonzalez

• 8:45 - 9:30   Molecular Replacement: Raji Edayathumangalam

• 9:30 - 10:15   Use of AI in Structural Biology: Dorothee Liebschner

• 10:15 - 10:30   Coffee Break

• 10:30 - 11:15   Studying Matalloenzymes with Complementary In-situ Spectroscopic Techniques: Aina Cohen

• 11:15 - 12:00   Introduction to Small Angle Scattering: Thomas Weiss

• 12:00 - 13:00   Lunch (Redwood rooms patio)

• 13:30 - 15:30   LCLS Tour

• 15:30 - 15:45   Coffee Break

• 16:00 - 18:00   Tutorials

• 18:30 - 19:30   Dinner (Redwood rooms patio)

• 19:45 - 20:45   Guest Lecture: TBA

Thursday 7 May

All day concurrent beamline and software tutorials

• 8:00 - 10:00   Tutorials

• 10:00 - 10:30   Coffee served in BL9-2 longe area

• 10:30 - 12:30   Tutorials

• 12:45 - 13:45   Lunch (Redwood rooms patio)

• 14:00 - 16:00   Tutorials

• 16:00 - 16:30   Coffee served in BL9-2 longe area

• 16:30 - 18:30   Tutorials

• 18:45 - 19:45   Dinner (Redwood rooms patio)

• 19:45 - 20:45   Guest Lecture: Brian Kobilka

Friday 8 May

All day concurrent beamline and software tutorials

• 8:00 - 10:00   Tutorials

• 10:00 - 10:30   Coffee served in BL9-2 longe area

• 10:30 - 12:30   Tutorials

• 12:45 - 13:45   Lunch (Redwood rooms patio)

• 14:00 - 16:00   Tutorials

• 16:00 - 16:30   Coffee served in BL9-2 longe area

• 16:30 - 18:30   Tutorials

• 18:45 - 19:45   Dinner (Redwood rooms patio)

• 19:45 - 20:45   Guest Lecture: TBA

Hands-on Sessions (1, 2, 3 and 4)

On Monday, as part of the "From Gene Synthesis to Crystallization" workshop we will offer the following tutorials:

• Use of the Gryphon robot to setup sitting drop vapor diffussion plates: Gabrielle Budziszewski
• Imaging with ARI system: Crissy Tarver
• Sample preparation for in situ-crystallization and robotic exchange during diffraction data collection: Aina Cohen
• Trace fluorescent labeling: Marc Pusey
• Phase diagram with the Oryx crystallization robot: Patrick Shawn Steward
• Hanging drop vapor diffusion plates setup: Darya Marchany
• Batch crystallization and sample embedding for XFEL experiments: LCLS staff

NX client installation and remote desktop workshop

On Tuesday, there will be a workshop to install and configure the NX client in the participants' laptops, and to learn how to access the data and demos in the practical tutorials.

Orientation to experiments at SSRL

On Tuesday after the afternoon lectures, there will a compulsory orientation to experiments at SSRL, covering safety, at building 120. There will be two talks:

• 15:30: Safety talk (Last name starting with A-L)

• 16:00: Safety talk (Last name starting with M-Z)

LCLS Tour

Sign up sheets for the optional tour of the free electron laser facility LCLS will be available on Tuesday. Groups will set off from building 48 (there is a ~15 minute walk to the LCLS). The tour will include a visit to the beamlines CXI and MFX, used for macromolecular femtosecond crystallography. The tour takes about 45 minutes.

Beamline Tutorials

We will offer tutorials for sample preparation, cryo-cooling, mounting and characterization; SAD/MAD data collection; RT data collection; crystal dehydration and UV-Vis spectrophotometry. All the participants will get the chance to learn how to collect data from their own samples or standard samples.

• Data collection on BL12-1: Irimpan Mathews and Darya Marchany-Rivera
• Data collection on BL12-2: Clyde Smith and Silvia Russi
• MAD/SAD data collection on BL14-1: Ailiena Maggiolo
• Sample preparation and harvesting tips and tricks on BL9-2: Jim Pflugrath, Elspeth Garman and Tzanko Doukov
• UV-Vis Microspectrophotometer introduction on 9-2: Aina Cohen
• RT data collection/crystal dehydration: Silvia Russi and Darya Marchany-Rivera

The participants must sign-up in advance for the tutorials they wish to attend. Instructions on how to access the sign-up sheets will be given during the course.

Software Tutorials

Software tutorials will be offered on several different packages for data processing and structure solution, including:

• Structure solution and refinement with Phenix: Li-Wei Hung, Pavel Afonine, Nigel Moriarty, Oleg Sobolev, Billy Poon and Dorothee Liebschner
• Data processing and phasing with CCP4: Harry Powell and Andrey Lebedev
• Molecular Replacement: Raji Edayathumangalam
• SHARP/autoSHARP, autoPROC, STARANISO, BUSTER: Clemens Vonrhein and Claus Flensburg
• Data processing with XDS: James Holton
• Data processing with DIALS: Aaron Brewster and Nick Sauter
• Data processing with HKL: Dominika Borek
• Coot: Paul Emsley
• SAXS data analysis: Tsutomu Matsui and Thomas Weiss

The participants must sign-up in advance for the tutorials they wish to attend. Instructions on how to access the sign-up sheets will be given during the course.