Note: This schedule is subject to changes.
All lectures on Sunday, Monday and Tuesday will take place in the Redwood rooms (building 48). Lunch and dinner will also be served in building 48, unless noted otherwise. The practical tutorials will take place at different locations around the SSRL and experimental floor.
See a map of SLAC indicating the main course venues.
Sunday 5 May
8:30 - 9:30 Registration (Redwood rooms, building 48)
9:30 - 12:00 Optional concurrent session 1: Crystallography 101: Bob Sweet (Redwood rooms C/D)
 - Waves and diffraction
 - Bragg’s Law and reciprocal space
 - Detection of X-rays
9:30 - 12:00 Optional concurrent session 2: Crystallography 1-on-1: Sample data collection strategy discussions with SSRL beamline and scientific staff (Redwood rooms A/B)
12:00 - 13:00 Lunch
13:00 - 15:30 Optional concurrent session 1:Crystallography 101: Bob Sweet (Redwood rooms C/D)
 - Fourier synthesis and structure
 - Space groups and crystal systems
 - Solving the phase problem
13:00 - 15:30 Optional concurrent session 2: Crystallography 1-on-1: Sample data collection strategy discussions with SSRL beamline and scientific staff (Redwood rooms A/B)
15:30 - 16:00 Coffee Break
16:00 - 17:00 NX Client installation and remote desktop workshop: Edgar Estebanez (Redwood rooms C/D)
17:30 - 19:30 Welcome Social (Stanford Guest House patio)
Monday 6 May
Lectures on crystal handling, data collection, detectors and data reduction (Redwood rooms C/D)
7:30 - 8:30   Registration (Redwood Rooms)
8:30 - 9:00   Introduction to RapiData: Clyde Smith
9:00 - 9:45   Macromolecular Cryocrystallography: Some opinions about best practices: Jim Pflugrath
9:45 - 10:30   Room temperature data collection: Silvia Russi
10:30 - 10:45   Coffee break
10:45 - 11:30   Radiation damage: James Holton
11:30 - 12:15   Getting the best data from photon counting detectors: Andreas Foerster
12:15 - 13:30   Lunch
13:30 - 14:15   Data processing fundamentals: Harry Powell
14:15 - 15:00   Data collection at Free Electron Lasers: Nick Sauter
15:00 - 15:15   Coffee break
15:15 - 16:00   Cryo-EM: Wah Chiu
16:00   Group photo (Redwood room patio)
16:30 - 17:30   Orientation to experiments /SSRL Tour (building 120)
18:00 - 18:45   Introduction to the tutorials (Redwood room C/D)
18:45 - 19:45   Banquet dinner (Redwood room patio)
19:45   Flash introduction of the participants (Redwood room patio or inside)
Tuesday 7 May
Lectures on structure solution and complementary methods (Redwood rooms C/D)
8:30 - 9:00   Why solve structures?: Art Lyubimov
9:00 - 9:45   SAD and MAD: Ana Gonzalez
9:45 - 10:30   Molecular Replacement: Raji Edayathumangalam
10:30 - 10:45   Coffee Break
10:45 - 11:30   SHELX: Andrea Thorn
11:30 - 12:15   Data processing and analysis for experimental phasing with SHARP/autoSHARP: Clemens Vonrhein
12:15 - 13:30   Lunch
13:30 - 14:15   Solve and Phenix: Dorothee Liebschner
14:15 - 15:00   In situ optical spectroscopy: Aina Cohen
15:00 - 15:30   Coffee break and tutorial sign-up
15:30 - 16:15   Introduction to Small Angle Scattering: Thomas Weiss
16:30 - 18:30   LCLS Tour
18:45 - 19:45   Dinner (Redwood rooms patio)
20:00 - 22:00   Tutorials/Sample Transfer tutorial
Wednesday 8 May
All day (8:00-22:00): Concurrent data collection and tutorials (see schedule below)
10:30   Coffee served in BL9-2 longe area
13:00 - 14:00   Lunch (Redwood rooms patio)
15:30   Coffee served in BL9-2 lounge area
17:00 - 18:00   Guest lecture: Brian Kobilka (Redwood rooms C/D)
Thursday 9 May
All day (8:00-22:00): Concurrent data collection and software tutorials (see schedule below)
10:30 Coffee served in BL9-2 lounge area
13:00 - 14:00   Lunch
15:30   Coffee served in BL9-2 lounge area
17:00 - 18:00   Guest lecture: James Fraser (Redwood rooms C/D)
Friday 10 May
10:00-11:00 Recapitulation and final discussions The ending time is approximate (Building 48)
On Sunday, there will be a workshop to install and configure the NX client in the participants' laptops, and to learn how to access the data and demos in the practical tutorials.
Participants who do not attend the Sunday course and need help with the NX client, can contact the organizers to arrange a demo.
On Monday 6th, after the afternoon lectures, there will a compulsory orientation to experiments at SSRL, covering safety, at building 120. There will be two talks:
On Tuesday 7th, between 20:00 and 22:00, we will do a demo on transferring frozen samples in cryovials to robot cassettes at the sample loading stations on BL9-2 and BL12-2. Participants who have brought frozen samples in cryovials or with unfrozen samples (in crystallization plates or capillaries) will get the chance to transfer them to SSRL cassettes after the demo.
Sign up sheets for the optional tour of the free electron laser facility
LCLS will be available on Monday. Group will set off from building
48 (there is a ~15 minute walk to the LCLS). The tour will include
a visit to the beamlines CXI and MFX, used for macromolecular femtosecond crystallography. The tour takes about 45 minutes.
During the data collection tutorials, the participants will learn how to optimize the data collection parameters for different experimental purposes.
We will offer tutorials for sample preparation, freezing, mounting and characterization; SAD/MAD data collection; RT data collection; crystal dehydration and UV-Vis spectrophotometry. All the participants will get beamtime to learn how to collect data from their own samples or standard samples. (See beamtime schedule below). For the participants interested in the MAD/SAD, RT, crystal dehydration, UV-Vis spectrophotometry and sample preparation tutorials, there will be sign-up sheets at the Redwood rooms on Tuesday during the afternoon coffee break and in the BL9-2 lounge area after that.
The slots marked as 'Spare' in the beamtime schedule can be used for participants to collect data by themselves, once they have been trained on data collection during a previous tutorial. Please see the organizers during the course if you need spare time for data collection or to ask about the possibility to collect data remotely after the course.
Time | Data collection at BL12-2 | RT data collection/Crystal dehyration/UV-Vis Microspectrophotometer (BL9-2) | MAD/SAD data collection (BL14-1) | Data collection at BL9-2 | Sample preparation (BL7-1) |
---|---|---|---|---|---|
Tues 20:00 | Unavailable | Unavailable | Unavailable | Unavailable | Sign-up |
Wed 8:00 | Haruehanroengran | Unavailable | Sign-up | Bastos/Mazurkewich/Wang/Yang/Edjer | Sign-up |
Wed 10:30 | Lei/McQuirter | Unavailable | Sign-up | Meng/Lanz/DeMaria/Hellmich/Kanchugal | Sign-up |
Wed 14:00 | Lee | Unavailable | Sign-up | Nepomuceno/Kluza/Wasserman/Arturo/Kashipathy/Torgeson | Sign-up |
Wed 19:00 | Sigh | Sign-up | Sign-up | Unavailable | Sign-up |
Wed 21:30 | Spare | Unavailable | Unavailable | Spare | Unavailable |
Thu 8:00 | Santiago/Stark | Unavailable | Sign-up | Ortolani/Soh/Carrioza/Escudero/Banjara/D'Agostino | Sign-up |
Thu 10:30 | Nemetcheck/Johnston | Unavailable | Sign-up | Thorsen/Velez/Mueller/Molt/Vanson/Brousseau | Sign-up |
Thu 14:00 | Hansen/Syrenne | Unavailable | Sign-up | Cheng | Sign-up |
Thu 19:00 | Tamura | Sign-up | Sign-up | Unavailable | Sign-up |
Thu 21:30 | Spare | Unavailable | Spare | Spare | Unavailable |
See A map of the SSRL beamlines.
Tutors
Software tutorials will be offered on several different packages for data processing and structure solution, including:
The participants must sign-up in advance for the software tutorials they wish to attend. There will be sign-up sheets at the Redwood rooms on Tuesday during the afternoon coffee break and in the BL9-2 lounge area after that.
Reservations can be made by phone (650) 926-2800, email guesthouse@stanford.edu
or via their web
site.
Course dates: May 5-10 2019
Applications opens: Oct 19 2018
Application deadline: Dec 31 2018
Applications received after the deadline will be
placed in a stand-by list.
Clyde Smith
Bus: (650)
926-8544
Email: csmith@slac.stanford.edu
Silvia Russi
Bus: (650) 926-5594
Email: srussi@slac.stanford.edu
Jeney Wierman
Bus: (650) 926-8682
Email: jwierman@slac.stanford.edu