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Equipments for B-SAXS/D Experiments on BL 4-2
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Flat window solution
cells: Type I (lexan) and Type II (15 µl sample volume cell made of black Delvin for photo-induced time-resolved experiments) |
Multiple solution
cell holder (automation of the sample translation can be synchronized with the data collection) |
Multiple solution
cell holder mounted in the solution scattering setup |
0 degree cryostat sample cell holder |
Closeup of 0 degree
cryostat sample cell holder |
0 degree cryostat |
In-vacuum cell
Newport Kinematic Base for Sample x,y Translation Stage
All B-SAXS sample holders attach to a Newport locking kinematic base which is mounted on an x,y translation stage. Users wishing to bring their own sample holders should make their designs compatible with this arrangement. The distance from the top of the base plate to the sample should be approximately 15.2 cm. The final sample position can be adjusted using the motorized x,y translation stage (± 5.00 mm vertical, ± 110.00 mm horizontal). The base is 3" x 3", with a 3 x 3 M5 tapped holepattern. The tapped holes are 1" apart from each other.
For ordering information and dimensions of the BKL-4M or BKL-4 kinematic base, contact Newport
B-SAXS/D Stopped-flow Mixers for Time-resolved Solution X-ray Scattering
• a Unisoku stopped-flow with optional optical monitoring capability
References for stopped-flow systems:
Unisoku stopped-
flow system, showing the two solution reservoirs and the active valves in the frontSchematic of the new Unisoku stopped-flow system. This version has improved features over the older one, including UV/vis compatibility, a passage for simultaneously monitoring local conformational change with x-ray scattering, and active valves that isolate the solution reservoirs to prevent solution back flow. "Stopped-Flow Apparatus for X-ray Scattering at Subzero Temperature" H. Tsuruta, T. Nagamura, Z.-X. Wang, K. Kimura, Y. Igarashi, A. Kajita, Y. Amemiya, K. Wakabayashi, H. Kihara Rev. Sci. Instrum. 60, 2356-2358 (1989).
"Stopped-flow apparatus for x-ray scattering and XAFS" H. Kihara, J. Synchrotron Radiation 1, 74-77 (1994).
When operated as a flow cell, the sample syringe can be emptied at a rate of 0.125mm/s. For a 1 ml syringe, 0.84 ml of protein can be used at a rate of 2.1 ml/s. For a 250 ml syringe, 210 ml of protein solution can be used at a rate of 0.525 ml/s. A slow-mixing cell can be attached to the flow cell for time-resolved studies in time scales of greater than ten seconds. Again, the dispensing rates will depend on the size of the syringes.
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The Flow Cell / Slow-mixing
Cell |
An XIA Shutter/filter assembly containing a two blade Tantalum shutter. There are ~0.08mm and 0.16mm Al filters for ~50% and 20% transmission, respectively. A second filter slot could accomodate an additional 1" square foil.
The XIA shutter/filter
assembly |
B-SAXS/D Minature Ionization Chamber
A minuature short-path ionization chamber for recording beam flux (Tsuruta-Cooper design). Kapton foil can be replaced with a thin mica window. Different versions of this ion chamber with different casing are in wide use at other SSRL stations.
A minuature short-path
ionization chamber for recording beam flux |
The ionization chamber,
from the opposite side (vacuum side) |
Photodiode Intensity Monitor / Beamstop
An IRD, Inc. photodiode has been incorporated into the beamstop located just upstream of the detector. This allows recording of the intensity of the x-rays transmitted through the sample and can also help with sample alignment.
A
photodiode / beamstop assembly |
The photoamplifier used with the photodiode / beamstop assembly was designed in-house by Vladimir Vinetskiy. The photoamplifier consists of two units, the preamplifier and the controller. Further information.
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