Bio SAXS Rapid Access Application

BL4-2 Bio-SAXS Rapid Access Application Form

One or two blocks of three shifts, including first shift for change-over and setup, are reserved for Rapid Access beam time each month. Applications are reviewed by SSRL's SMB & Biophysics Proposal Review Panel for scientific merit and feasibility.
Rapid Access will in general only be granted once for a user group during a single scheduling period.
Only one project per Principal Investigator may be scheduled for Rapid Access time within a given month. If multiple spokespersons in a group submit applications, the PI will be asked to set priority for scheduling the experiments.
Spokespersons should expect to hear back from SSRL within two weeks of the deadline for submittal. If the application has been approved with the highest ranking among the submissions for a given deadline, beam time will be assigned within one month of the original submittal. If the application has been approved for beam time but assigned a lower ranking, it will be put on a waiting list for beam time during the next block of Rapid Access time or for consideration to fill cancellations of regularly scheduled beam time.
Use Agreement(s) must be fully executed between Stanford University and the spokesperson's home institution and all collaborator institutions (if personnel physically coming to SSRL) before beam time.

Spokesperson:	Institution:	Email:	Degree:

	Work Phone:	Fax:

Principal Investigator:	Email:	Work Phone:


Collaborators:	Institution: (if different from above)	Email:	Degree:


Title:


Scientific Importance: (8-10 lines) State clearly what you hope to achieve and why this project is important. Why will these results be important? What are reasons for rapid access?


Are samples currently ready, or can be prepared with 1-2 week notice? Yes No

Feasibility: (8-10 lines) Projects will only be considered for Rapid Access time if well-characterized samples are currently ready for data collection or can be made so with short notice. The extent of characterization will depend upon specific details of the project, but would usually be performed using complementary methods such as light scattering, gel filtration, gel electrophoresis. State clearly what samples are currently ready for data collection, to what extent characterization has been done for these samples, what data you expect to obtain, and how the data will be used (e.g. verifying crystallographic quaternary structure).


Previous Rapid Access: (8-10 lines) Fill out this section if Rapid Access time was previously awarded to the same PI during the last 12 months. Only describe the most recent Rapid Access data collection if more than one was allocated during this period. Briefly state what data were collected and their quality. What were the scientific goals and were they met -- explain how the experiment was successful or why the effort failed.




AIDS Related? Yes No

Other Disease? Yes No If yes, please specify:

Private Sector Research? Yes No Note: Private Sector Research is subject to specific terms and conditions, and SSRL must be reimbursed at full cost recovery.

Source of Research Funds: (hold Ctrl to select multiple options)		Research Area: (hold Ctrl to select multiple options)

Type of measurement:
Solution scattering (Gas detector or Image Intensified CCD)
Fiber/lipid/membrane diffraction (Image Intensified CCD or MarCCD)

Note: Time-resolved measurements will not be supported.

Sample Data:

Sample Stable Concentration Range
(mg/ml) Mol. Wt.
(kD) Available Sample Quantity (mg) Content of Buffer Solution

Expected Radius of Gyration (Å)
in case of solution scattering Temp. for
Data
Collection
Lowest Angular Scale Desired Highest Angular Scale Desired

2*sin(q)/l
4*pi*sin(q)/l Å^-1
nm^-1

SMB Sample Preparation Lab Equipment Required:

Ultracentrifuge EPR Spectrophotometer Wetlab Bench Space

Glove Box Glove Bag Fume Hood Cold Room

Sample Cells Required:

Standard polycarbonate solution cell

(Please check with bioSAXS/D staff regarding
the preparation of solution cells.)

Stopped-flow ans syringe mixer No support required (own equipment)

(Sample holder must fit the standard Newport kinematic mount on the 4-2 sample stage. Check bioSAXS/D web page for further information)

Additional Requirements:
Please note that due to the limited amount of beam time available for Rapid Access, it may not be possible to fully accommodate a user's experimental requirements. However, if there are additional requirements that would further enable the success of your experiment, please use the space provided below to describe those requirements. Please feel free to contact Hiro Tsuruta (tsuruta@slac.stanford.edu) for questions and consultation regarding your experiment.

Safety Concerns:

CHEMICAL USE? yes no
If yes, list substances (separate by comma) and complete a hazard form(s) for each substance.

Substance(s)

Common Name(s)

Chemical Formula(s)

Total Amount(s)

Form
(solid, liquid,
gas, powder)

NANOSCALE MATERIALS USE? yes no
If yes, will there be open manipulation of nanoscale material samples at SSRL? yes no
If no, how are the samples contained?
If yes, a safety plan must be submitted to the SSRL Safety Office for approval. See CDC/NIOSH website for guidance. http: //www.cdc.gov/niosh/topics/nanotech/safenano/

BIOHAZARDOUS MATERIALS USE? yes no
If yes, what type and what is the materials NIH classification?

HUMAN OR ANIMAL SUBJECT USE? yes no
If yes, what type?
*Please contact SSRL Safety Office for further guidance.

RADIOACTIVE MATERIALS USE? yes no
If yes, what is the materials specific activity?

*Radioactive substances may not be brought directly to SSRL. They first must be shipped or taken to Operational Health Physics, Bldg. 24, MS 84, 2575 Sand Hill Rd., Menlo Park, CA 94025; please contact Carol Morris at 650-926-3023 or Jim Allen at 650-926-4064.

LASER USE yes no

If yes, please fill out all of the fields in this section.

ANSI classification

Wavelength

Total Power

Laser hazard controls you will apply.

HAZARDOUS EQUIPMENT/ELECTRICAL EQUIPMENT? yes No
If yes, describe any hazardous/electrical equipment you will be bringing to SSRL. Indicate if it is a commercial product and if it has been altered in any way.

Electrical equipment brought with you for use at SSRL must be certified by a Nationally Recognized Testing Laboratory. If your equipment is not certified, please contact the SSRL Safety Office for guidance on obtaining certification for your equipment under SLAC's Electrical Equipment Inspection Program (EEIP).

SSRL User Administrative contact: Cathy Knotts, knotts@slac.stanford.edu, tel. 650-926-3191