Laboratories Available to Users
Structural Molecular Biology Sample Preparation Laboratories
(Bldgs.
120 and 131)
The SSRL Structural Molecular Biology (SMB) Sample Preparation Laboratories are
available to users by prearrangement. To use a laboratory, you must complete laboratory training, and provide a list of all chemicals
that you are bringing to use or store in the Sample Preparation
Laboratory. A list
of chemicals available in the Laboratories is also attached.
Lab space is assigned by Cynthia Patty (650-926-3925). Users should him before
setting up equipment or beginning work in the lab.
The following equipment is available in the Sample Preparation
Laboratories:
Building 120 Laboratory:
- Hewlett-Packard UV-VIS diode-array spectrophotometer model #8452A with
a 89090A Peltier temperature control unit. Wavelength range 190-820 nm with a
2-nm optical bandwidth. Temperature range 10-70°C. Disposable plastic
cuvettes and, on a sign-out basis, quartz and glass cuvettes are available.
- Beckman GPKR centrifuge, 8000 rpm max. Fixed angle rotor is available.
Walk-in 4°C cold room with a Wild M3Z stereo microscope, a pH meter
with Ag/AgCl semi- and micro-glass electrodes.
- Vacuum Atmospheres HE-43-2-NEX glove-box (including an oxygen analyzer)
with nitrogen atmosphere for inert atmosphere (oxygen and moisture free) work.
Open handling of volatile solvents within the box is forbidden. There is a
Mettler PR203 balance (0-210g±0.001g/±0.005g) permanently in the box.
- Jeol JES-RE1X X-band EPR spectrometer with an Oxford cryostat.
Available after required training has been completed.
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Building 131 Laboratory:
- Beckman L5-65 ultracentrifuge, 1000-65,000 rpm. Swinging bucket rotor
SW55Ti with buckets are available.
- Vacuum Atmospheres HE-493 glove-box (including a Vacuum Atmospheres
oxygen analyzer) with nitrogen atmosphere for inert atmosphere (oxygen and
moisture free) work. Open handling of volatile solvents within the box is
forbidden. There is a Mettler PM460 balance (0-410 g±0.001 g/±0.0l g)
permanently in the box.
- Precision Scientific convection oven with maximum temperature of 225°C.
- Walk-in 4°C cold room with refrigerated Fisher Marathon 21K/Br
centrifuge (0-13,300 rpm dependent on rotor), pH meter model a Ag/AgCl glass
electrode and a micro electrode.
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Both Laboratories:
- pH meters with Ag/AgCl semi- and micro-glass electrodes. Standard
buffers are available.
- Stereomicroscope with adjustable lenses, polarized light attachment,
rotational stage and crystal alignment stage.
- Mettler PC440 balance for the range 0.001-400 grams. Reproducibility
±0.001 grams up to 40 grams; ±0.01 grams above that.
- Mettler AG104 analytical balance, range 0.001-101g. Repeatability ±0.1
mg.
- Millipore water purifier, type IV 18 megaohm.
- Ice machines, refrigerator/freezer for temporary storage of chemicals
and biological samples, dishwasher, limited glassware, common chemicals and
other general laboratory equipment such as microfuge with microtubes, vortex
mixer, ultrasonic cleaning bath, magnetic stirrers, hot plates, etc.
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Most instruments have instructions for their use. Instructions can also be
mailed to you prior to your visit if requested. [Cynthia Pattty,
cpatty@SLAC.Stanford.EDU, (650) 926-3925]
NO EQUIPMENT SHOULD BE REMOVED FROM THE SMB
LABS
User Machine Shop
The Building 120 Machine Shop Rules and Requirements document must be
read and the Certification of Personal Responsibility must be signed and
turned in to the custodian or duty operator before using any machine or tool in
the shop.
Sign out all tools before removing them from the shop. The custodian
of the User Machine Shop is Charles Weidner (ext. 2029). Please clean
up after yourself.
User Vacuum Shop
To use the User Vacuum Shop contact Curtis Troxel
(ext. 3870).
