EXPERIMENTAL STATION 9-3

CURRENT STATUS:	Open
SUPPORTED TECHNIQUES:	X-ray absorption spectroscopy Single crystal x-ray absorption spectroscopy X-ray absorption spectroscopy imaging
MAIN SCIENTIFIC DISCIPLINES:	Structural Biology
% TIME GENERAL USE:	100%
SCHEDULING:	see Proposal Submittal and Scheduling Procedures
SOURCE:	16-pole, 2-Tesla wiggler, 2 mrad beam
BEAM LINE SPECIFICATIONS:   energy range grating type resolution DE/E spot size (fwhm) flux* angular acceptance focused 5000-30000 eV Si(220) 1 x 10-4 0.4 x 3 mm2 ~2 x 1012 2.0 mrad unfocused 5000-30000 eV Si(220) 1 x 10-4     2.0 mrad *ph/sec @100 mA / 9 keV w 1x4 mm aperture
OPTICS:	M0 mirror: Flat, bent, vertically collimating, 1 m, Si, Rh-coated, LN2-cooled                 monochromator M1 mirror: Bent, cylindrical, 1.2 m, Zerodur, Rh-coated
MONOCHROMATOR:	Si(220) phi=0°, Si(220) phi=90° double-crystal, non-fixed exit slit
ABSORPTION:	546 microns Be, 16.0 meters He, 15.2 microns C
INSTRUMENTATION:	30-element Ge detector array LHe cryostat (4-200 K) Ion chambers, PIPS detector Huber kappa diffractometer, MAR IP or CCD detector for single crystal XAS Capillary-based micro-imaging instrumentation LHe Cryosystems cryocooler for single crystal & imaging
DATA ACQUISITION AND ANALYSIS:	Data acquisition: XAS-Collect Data analysis: EXAFSPAK
RESPONSIBLE STAFF:	Matthew Latimer, 650-926-4944 (office), 650-714-9416 (cell) Allyson Aranda, 650-926-3938 (office), 650-714-9415 (cell) Erik Nelson, 650-926-5103 (office), 650-714-9407 (cell) Serena DeBeer George, 650-926-4674 (office)
BEAM LINE PHONE NUMBER:	650-926-5293
GENERAL DESCRIPTION:	BL9-3 is dedicated to biological x-ray absorption spectroscopy. Three main types of experiments can be performed: XAS of very dilute (sub-millimolar) solutions of metalloproteins, polarized single crystal XAS studies, capillary based microXAS imaging experiments. It is equipped with a 30-element Ge detector array and a LHe cryostat when used for solution XAS studies. A combined XAS/crystallography instrument focusing on single crystal XAS, is based on a kappa goniometer, a MAR165 CCD camera or MAR145 IP detector, a LHe cryostream sample cooler and specialized software. Instrumentation and software for capillary-based micro-focus XAS imaging of biological specimens is available, and includes a high-precision stage and a Vortex drift diode detector.
SCIENTIFIC APPLICATIONS / SELECTED RESULTS:	November 2007: Junko Yano Wins Robin Hill Award August 2007: The Chemical Form of Mercury in the Fish We Eat April 2007: Zebra Fish in the Mineshift: Understanding Mercury Toxicity January 2007: A New Slant on a Cellular Balancing Act - the Copper-sensing Repressor of Mycobacterium tuberculosis November 2006: Where Water is Oxidized to Dioxygen: Structure of the Photosynthetic Mn4Ca Cluster September 2006: A Fern Fatale - X-ray Absorption Spectroscopy Imaging of an Arsenic-Loving Fern July 2006: An Octahedral Coordination Complex of Iron(VI) February 2006: Structural Insights into FeMo Cofactor Biosynthesis December 2005: Reexamination of Lead(II) Coordination Preferences in Sulfur-Rich Sites: Implications for a Critical Mechanism of Lead Poisoning September 2005: Damage by X-rays: A Case Study for Metallo-Protein Crystallography June 2005: Characterization of a Genuine S=1/2 Fe(V) Complex April 2004: Fighting Antibiotic Resistance The dapE-encoded N-succinyl-L,L-Diaminopimelic Acid Desuccinylase from Haemophilus influenzae is a Dinuclear Metallohydrolase
ENGINEERING NOTES:	http://www-ssrl.slac.stanford.edu/safety/beamlines/bl9/

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Last Updated:	22 APR 2008
Content Owner:	Britt Hedman
Page Editor:	Nikola Stojanovic