EXPERIMENTAL STATION 9-3

CURRENT STATUS:	Closed (Down-pending mirror repair)
SUPPORTED TECHNIQUES:	X-ray absorption spectroscopy Single crystal x-ray absorption spectroscopy
MAIN SCIENTIFIC DISCIPLINES:	Structural Biology
% TIME GENERAL USE:	100%
SCHEDULING:	Proposal Submittal and Scheduling Procedures Current SPEAR and Beam Line Schedules
SOURCE:	16-pole, 2-Tesla wiggler, 2 mrad beam
BEAM LINE SPECIFICATIONS:   energy range resolution DE/E spot size (fwhm) flux* angular acceptance focused 5000-30000 eV 1 x 10-4 0.4 x 3 mm2 ~2 x 1012 2.0 mrad *ph/sec @100 mA / 9 keV w 1x4 mm aperture
OPTICS:	M0 mirror: Flat, bent, vertically collimating, 1 m, Si, Rh-coated, LN2-cooled monochromator M1 mirror: Bent, cylindrical, 1.2 m, Zerodur, Rh-coated
MONOCHROMATOR:	Si(220) phi=0°, Si(220) phi=90° double-crystal, non-fixed exit slit Monochromator Crystal Glitch Library Crystal changes need to be scheduled and coordinated in advance with BL support staff.
ABSORPTION:	768 microns Be, 205 microns C
INSTRUMENTATION:	30-element Ge detector array LHe cryostat (4-200 K) Ion chambers, PIPS detector Huber kappa diffractometer, MAR IP or CCD detector for single crystal XAS LHe Cryosystems cryocooler for single crystal & imaging
DATA ACQUISITION AND ANALYSIS:	Data acquisition: XAS-Collect Data analysis: EXAFSPAK X-ray Data Booklet
RESPONSIBLE STAFF:	Matthew Latimer, 650-926-4944 (office), 650-714-9416 (cell) Erik Nelson, 650-926-5103 (office), 650-714-9407 (cell) Ritimukta Sarangi, 650-926-4621 (office)
BEAM LINE PHONE NUMBER:	650-926-5293 On-site Users: Contact the Duty Operator at 9-926-4040
GENERAL DESCRIPTION:	BL9-3 is dedicated to biological x-ray absorption spectroscopy. Three main types of experiments can be performed: XAS of very dilute (sub-millimolar) solutions of metalloproteins, polarized single crystal XAS studies. It is equipped with a 100-element Ge detector array and a LHe cryostat when used for solution XAS studies. A combined XAS/crystallography instrument focusing on single crystal XAS, is based on a kappa goniometer, a MAR165 CCD camera or MAR145 IP detector, a LHe cryostream sample cooler and specialized software.
Sample Preparation Labs:	The SSRL Structural Molecular Biology (SMB) Sample Preparation Laboratories are available to users by prearrangement. To use a laboratory, users must complete laboratory training, and provide a list of all chemicals to be brought, used or stored in the Sample Preparation Laboratory. For details, contact Cynthia Patty (650-926-3925).
SCIENTIFIC APPLICATIONS / SELECTED RESULTS:	February 2009: Localizing organomercury uptake and accumulation in zebrafish larvae at the tissue and cellular level November 2007: Junko Yano Wins Robin Hill Award August 2007: The Chemical Form of Mercury in the Fish We Eat April 2007: Zebra Fish in the Mineshift: Understanding Mercury Toxicity January 2007: A New Slant on a Cellular Balancing Act - the Copper-sensing Repressor of Mycobacterium tuberculosis November 2006: Where Water is Oxidized to Dioxygen: Structure of the Photosynthetic Mn4Ca Cluster September 2006: A Fern Fatale - X-ray Absorption Spectroscopy Imaging of an Arsenic-Loving Fern July 2006: An Octahedral Coordination Complex of Iron(VI) February 2006: Structural Insights into FeMo Cofactor Biosynthesis December 2005: Reexamination of Lead(II) Coordination Preferences in Sulfur-Rich Sites: Implications for a Critical Mechanism of Lead Poisoning September 2005: Damage by X-rays: A Case Study for Metallo-Protein Crystallography June 2005: Characterization of a Genuine S=1/2 Fe(V) Complex April 2004: Fighting Antibiotic Resistance The dapE-encoded N-succinyl-L,L-Diaminopimelic Acid Desuccinylase from Haemophilus influenzae is a Dinuclear Metallohydrolase August 2003: X-ray Absorption Spectroscopy Catches the Chemical Form of Mercury in Fish
ENGINEERING NOTES:	http://www-ssrl.slac.stanford.edu/safety/beamlines/bl9/

SSRL | Beamlines | User Admin | Safety

Last Updated:	28 JAN 2011
Content Owner:	Matthew Latimer
Page Editor:	Nikola Stojanovic