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Phase Contrast While some specimens of biomedical interest, such as bone, do show amplitude, i.e., absorption, contrast with multi-keV X-rays, most specimens contain only low-Z elements, and are nearly impossible to study in amplitude contrast at these energies. It is well known, however, that phase contrast is very useful under these conditions. The physics behind this difference is that while the imaginary part of the atomic scattering factor f2 drops rapidly with energy, the real part, f1 approaches a constant, equal to the atomic number. It is essential to have a phase contrast mode of operation for bioimaging.Downloads & Links  If you would like to read the full research paper please click on the PDF icon to download the research paper that explores the new TXM imaging technology at SSRL. |
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Imaging Samples TXM 5x5 mosaic composite of osteoporotic mouse bone, with phase contrast at 8 keV. Osteoporosis was induced by hind-limb unloading, a NASA-developed-method to simulate microgravity effects in ground experiments. (Right) Averaged (20 1-sec scans) high-resolution image of lacuna from mosaic on the left.  Normal mouse tibia trabecular region in microCT at 10 µm resolution (left), followed by 20X objective differential interference contrast picture of a single thin trabecula, at ~ 0.75 µm resolution (center), followed by TXM mosaic composite with phase contrast at 8 keV, with 40 nm resolution (right). |
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